Review



elispot flex human igg alp  (Mabtech Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Mabtech Inc elispot flex human igg alp
    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG <t>ELISPOT</t> with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Elispot Flex Human Igg Alp, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+flex+human+igg+alp/antibody+biotinylated+ifn%CE%B3+specific/pmc12915279-60-0-6
    Average 86 stars, based on 1 article reviews
    elispot flex human igg alp - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Robust effector memory features of human T-bet hi B cells induced by repeated mRNA vaccination"

    Article Title: Robust effector memory features of human T-bet hi B cells induced by repeated mRNA vaccination

    Journal: iScience

    doi: 10.1016/j.isci.2026.114804

    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG ELISPOT with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Figure Legend Snippet: RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG ELISPOT with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.

    Techniques Used: Activation Assay, Fluorescence, FACS, In Vitro, Enzyme-linked Immunospot, Neutralization, Binding Assay, Inhibition, MANN-WHITNEY

    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Robust effector memory features of human T-bet hi B cells induced by repeated mRNA vaccination
    Article Snippet: Chromium Single Cell Human BCR Amplification Kit, 16 rxns , 10X Genomics , Cat# PN-1000253. .. ELISpot Flex: Human IgG (ALP) , MABTECH , Cat# 3850-2A. .. SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit , GenScript , Cat# L00847 -A.



    Similar Products

    elispot flex human igg alp  (Mabtech Inc)
    86
    Mabtech Inc elispot flex human igg alp
    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG <t>ELISPOT</t> with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Elispot Flex Human Igg Alp, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+flex+human+igg+alp/antibody+biotinylated+ifn%CE%B3+specific/pmc12915279-60-0-6
    Average 86 stars, based on 1 article reviews
    elispot flex human igg alp - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    elispot flex-human igg (alp) kit  (Mabtech Inc)
    90
    Mabtech Inc elispot flex-human igg (alp) kit
    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG <t>ELISPOT</t> with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Elispot Flex Human Igg (Alp) Kit, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+flex+human+igg+alp/elispot+flex++mouse+igg++alp++kit/pmc11680267-159-14-7
    Average 90 stars, based on 1 article reviews
    elispot flex-human igg (alp) kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    elispot flex-human igg (alp)  (Mabtech Inc)
    90
    Mabtech Inc elispot flex-human igg (alp)
    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG <t>ELISPOT</t> with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Elispot Flex Human Igg (Alp), supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+flex+human+igg+alp/elispot+flex++mouse+igg++alp++kit/pm39218373-131-1-6
    Average 90 stars, based on 1 article reviews
    elispot flex-human igg (alp) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    elispot flex: human igg (alp) kit  (Mabtech Inc)
    90
    Mabtech Inc elispot flex: human igg (alp) kit
    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG <t>ELISPOT</t> with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.
    Elispot Flex: Human Igg (Alp) Kit, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+flex+human+igg+alp/elispot+kit/pmc10826747__23___0542___Techapp___s1-44-17-23
    Average 90 stars, based on 1 article reviews
    elispot flex: human igg (alp) kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG ELISPOT with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.

    Journal: iScience

    Article Title: Robust effector memory features of human T-bet hi B cells induced by repeated mRNA vaccination

    doi: 10.1016/j.isci.2026.114804

    Figure Lengend Snippet: RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG ELISPOT with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.

    Article Snippet: ELISpot Flex: Human IgG (ALP) , MABTECH , Cat# 3850-2A.

    Techniques: Activation Assay, Fluorescence, FACS, In Vitro, Enzyme-linked Immunospot, Neutralization, Binding Assay, Inhibition, MANN-WHITNEY